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Mr. Freeze's next appearance is as the main antagonist in the direct to video movie Batman & Mr. Freeze: SubZero. After Nora's tank is shattered, Freeze kidnaps Barbara Gordon in order to perform a vital organ transplant and uses Barbara to get it. Freeze makes a deal with a greedy old colleague of his to help cure his wife in exchange for gold. Batman and Robin thwart this plan and Freeze is then presumed killed in the explosion of his lair on an abandoned oil rig. Wayne Enterprises then finances Nora's surgery saving her life which Freeze overhears while watching in on researchers in the North Pole, bringing tears to his eyes.

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